|Title:||Characterization of maltase and sucrase inhibitory constituents from Rhodiola crenulata||Authors:||Li, Wen-Tai
|Keywords:||maltase;sucrase;inhibitor;inhibition kinetics assay;Rhodiola crenulata||Issue Date:||2019||Journal:||Foods||Abstract:||The inhibitory properties of epicatechin-(4�],8)-epicatechingallate (B2-3��-O-gallate), epicatechin gallate (ECG), and epicatechin (EC) isolated from Rhodiola crenulata toward maltase and sucrase were investigated. The half-maximal inhibitory concentration (IC50) values for maltase were as follows: B2-3��-O-gallate (1.73 �� 1.37 �gM), ECG (3.64 �� 2.99 �gM), and EC (6.25 �� 1.84 �gM). Inhibition kinetic assays revealed the inhibition constants (Ki) of the mixed-competitive inhibitors of maltase, as follows: B2-3��-O-gallate (1.99 �� 0.02 �gM), ECG (3.14 �� 0.04 �gM), and EC (7.02 �� 0.26 �gM). These compounds also showed a strong inhibitory activity toward sucrase, and the IC50 values of B2-3��-O-gallate, ECG, and EC were 6.91 �� 3.41, 18.27 �� 3.99, and 18.91 �� 3.66 �gM, respectively. Inhibition kinetic assays revealed the inhibition constants (Ki) of the mixed-competitive inhibitors of sucrase as follows: B2-3��-O-gallate (6.05 �� 0.04 �gM), ECG (8.58 �� 0.08 �gM), and EC (13.72 �� 0.15 �gM). Overall, these results suggest that B2-3��-O-gallate, ECG, and EC are potent maltase and sucrase inhibitors.||Description:||Foods 8(11): pii: E540||URI:||http://localhost/handle/123456789/50||DOI:||10.3390/foods8110540|
|Appears in Collections:||李文泰|
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